MIR-448 Regulates MAGEA6/AMPK Signaling Pathway in Hepatocellular Carcinoma Tumor Stem Cells

Objective: To explore the role of miR-448 in regulating MAGEA6/AMPK signaling pathway biological study hepatocellular carcinoma (HCC) tumor stem cells. Methods: Using database, related expression chips were obtained and regulatory mirnas candidate genes predicted, predicted results analyzed. The effects MAGEA6 on pellet formation rate clone cells detected by immunofluorescence identification cell markers light microscope counting method. migration invasion scratch Transwell assay. Dual luciferase reporter assay to verify whether targets MAGEA6. influence AMPK qRT-PCR Western blot. Results: It was found that may directly regulate Overexpression inhibited characteristics, proliferation, migration, vitro, as well ability xenograft vivo. However, inhibition showed opposite results. In addition, regulates signaling. Silencing adding activator further verified activated targeting MAGEA6, thus affecting hepatoma Conclusions: Our reveal activates thereby is suggested overexpression be a new therapeutic strategy for carcinoma.